Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle. The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.
Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
Disodium p-nitrobenzene phosphate (p-NPP), a widely used phosphatase chromogenic substrate, can form p- nitrophenol under the action of acid phosphatase. Under alkaline conditions, p-nitrophenol is yellow and has a maximum absorption peak at 405 nm. The darker of the yellow product is, the higher of the ACP activity is. Therefore, the activity of ACP can be calculated by measuring the OD value at 405 nm.
Aconitase catalyzes isocitrate to produce cis-aconitase. Cis-aconitase has a characteristic absorption peak at 240 nm. The activity of this enzyme was calculated by measuring the production rate of cis-aconitase.
Acyl-CoA synthase (ACS) is an important enzyme that is widely present in living organisms and participates in the metabolic process of fatty acids. It is an enzyme necessary for lipid synthesis, fatty acid catabolism and membrane remodeling, and can also function as transcriptional activator, allosteric inhibitor or precursor of inflammatory mediators. In the medical field, mutations or abnormal expression of ACS may be associated with various diseases, such as diabetes and obesity. The detection principle of this kit: The ACS catalyzes the substrate, and the generated product reacts with the chromogenic agent to produce a purple substance, which has the largest absorption peak at 555 nm. Therefore, the absorbance at 555 nm can be measured to calculate the amount of product generated, thereby calculating the activity of ACS.
Adenosine is an endogenous nucleoside distributed throughout human cells. It can directly enter the myocardium and be phosphorylated to produce adenosine, which is involved in myocardial energy metabolism, and also participates in the dilation of coronary vessels and increase blood flow. Adenosine has physiological effects on the cardiovascular system and many other systems and tissues of the body. It also promotes the growth of human hair follicles and the proliferation of dermal papilla cells in vitro. The detection principle of this kit is that the substances produced by the enzyme-catalyzed adenosine reaction consume NADH, the OD value of the system decreases at 340 nm, and the content of adenosine can be calculated by calculating the decline rate at 340 nm.
Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
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