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This kit provides a fluorescent probe that can specifically bind to Fe2+, and the probe can enter the cell interior well, which is suitable for the detection of Fe2+ in alive cells. When the probe reacts with Fe2+, an irreversible orange (red) fluorescent product is generated (excitation wavelength: 542 nm emission wavelength: 575 nm).
GPX catalyzes the substrate, and the product consumes the reducing agent with the addition of enzyme reagents. The reducing agent has a maximum absorbance at 340 nm. The activity of GPX can be calculated by measuring the change of absorbance value at 340 nm.
Hydrogen Sulfide (H2S), as a key signal transduction gas molecule in cells and organisms, is involved in numerous physiological and pathological processes such as vascular relaxation and nerve transmission. The detection of intracellular H2S can deeply explore its specific mechanism and role in cellular signal transduction. Given that abnormal intracellular H2S concentration is associated with various diseases such as diabetes and liver cirrhosis, the detection of intracellular H2S can play an auxiliary role in the early diagnosis of diseases, condition monitoring, and evaluation of treatment effects. The detection principle of this kit: the probe produce green fluorescence with H2S after entering the cell. The level of H2S in cells can be detected by measuring the fluorescence intensity.
Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through the basic unit structures of the complex such as iron-sulfur proteins. Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.
Mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm.
Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The activity of mitochondrial complex I can be quantified by measure the change OD value at 340 nm.
Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
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