"Chitinase mainly exists in the shell of crustaceans such as shrimp, crabs, insects, organs of mollusks and cell walls of fungus. Chitinase can catalyze the hydrolysis of chitinase and has the function of resisting fungus infection, so it has become the research focus of anti-fungus diseases. This kit can calculate the activity of chitinase by measuring the OD value change at 585 nm in unit time of chitinase catalyzed chitinase hydrolysis products.
Chloride ion in biological fluids are replaced by the mercury ions in mercury thiocyanate through ion replacement, which resulted in the formation of difficult-to-dissociate mercury chloride. The substituted thiocyanate ions were combined with ferric nitrate to form a red complex. The content of chlorine ion can be calculated indirectly by measuring the OD value at 460 nm.
Cholesterol is the main steroid compound in mammals and plays an important role in basic cellular life activities. Moderate intake of cholesterol can provide a good guarantee for the oxidation stability and physical stability of liposomes, and has a preventive effect on cardiovascular diseases, but excessive intake of cholesterol has the risk of causing hyperlipidemia, tumor and other diseases. Therefore, it is of great significance to study the ability of cells to take up cholesterol. This kit can measure cholesterol uptake ability in cell samples by convenient fluorescence method. Cholesterol substrate in this kit can enter cells through the endocytosis mediated by low density lipoprotein receptor on the cell membrane, and the ability of cells to take up cholesterol can be measured by fluorescence microscopy, fluorescence microplate reader, flow cytometry and other instruments to detect fluorescence intensity.
Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems. Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.
Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
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