Ascorbic acid (AsA) is an important physiological index of plant cells. The changes of its content, redox status (AsA/DHA ratio), and the activities of enzymes related to its synthesis and metabolism are involved in the response of plants to a series of environmental stresses. Dehydroascorbic acid (DHA) is a reversible oxidized form of AsA. In vivo, dehydroascorbic acid (DHA) and ascorbic acid form a redox system and act as an electron acceptor. The detection principle of this kit is that DTT reduces DHA to produce AsA, and the DHA content can be calculated by measuring the formation rate of AsA in the system at 265 nm.
Diamine oxidase can catalyse amine substances to produce hydrogen peroxide, and hydrogen peroxide can react with the chromogenic substance to produce chromogenic substance, which has a characteristic absorption peak at 460 nm. The activity of diamine oxidase can be calculated by measuring the change rate of absorbance per unit time.
Dihydrofolate Reductase (DHFR) is one of the key enzymes in nucleic acid metabolism and the main target enzyme in cancer drug therapy, and has been widely studied in recent years. The detection principle of this kit is as follows: DHFR catalyzes substrate reactions to consume NADPH. NADPH has a characteristic absorption peak at 340 nm. The activity of DHFR can be characterized by measuring the rate of decrease in absorbance at 340 nm.
Dihydroxyacetone phosphate (DHAP) is an intermediate product present in the glycolysis and gluconeogenesis processes of living organisms. Most animals, due to the absence of the glyoxylate cycle, cannot convert fatty acids into glucose like plants. They can only convert the glycerol in fats into glucose through DHAP or enter the glycolysis pathway. Detection principle: The products formed after a series of enzymatic reactions of DHAP can cause the fluorescent probe to emit fluorescence. The fluorescence value can be detected using a fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm to calculate the content of DHAP.
Dipeptidyl Peptidase IV (DPP4), also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition.
The detection principle of this kit is that DPP4 can decompose the substrate and release fluorescent substance AMC. The addition of DPP4 inhibitor can inhibit the enzyme activity, and the inhibitory ability of the inhibitor can be determined by the fluorescence value.
Bilirubin is one of the important components of bile. It is the degradation product of hemoglobin in various heme proteins under the action of a series of enzymes. It is important to the digestion and absorption of lipids and the formation of yellow distemper. Bilirubin comes in two forms: water-soluble and water-insoluble. Bilirubin has powerful antioxidant, anti-inflammatory and autoimmune properties. The concentration of bilirubin in human body is related to sex, drug intake, age and so on. Low serum bilirubin is directly related to diabetes, metabolic syndrome, cardiovascular disease and other pathological states. However, high bilirubin is indicative of hemolysis, jaundice, Gilbert syndrome, hepatitis, drug toxicity, and possible bile duct obstruction.
Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A
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