Adenosine deaminase (ADA) can hydrolyzed the substrate adenosine to form hypoxanthine riboside, which is hydrolyzed by purine riboside phosphatase to produce hypoxanthine and phosphate ribose. Under the action of xanthine oxidase, hypoxanthine produces hydrogen peroxide, which produces red substance under the action of peroxidase, 4-aminotepyrine and color source. The red substance has the maximum absorption peak at 550 nm and the changes of absorbance is proportional to the activity of ADA.
Adenosine diphosphate (ADP) is a nucleoside that plays a key role in energy transfer reactions. In organisms, ADP is usually the product of Adenosine triphosphate (ATP) which hydrolysis after the loss of a phosphate group, that is breaking a high-energy phosphate bond and releasing energy. ADP exists in high-density particles in platelet cells and is released when platelets undergo coagulation reaction. ADP affects the shape and biological behavior of platelets through ADP receptors on platelets, and further accelerates the process of platelet coagulation. The detection principle of this kit is that ADP and the substrate are catalyzed by enzyme reagents to make the chromogenic agent produce fluorescence. The fluorescence microplate reader was used to detect the excitation wavelength of 535 nm and the emission wavelength of 587nm, and the ADP content in the sample was calculated according to the standard curve.
Adenosine diphosphate glucose pyrophosphorylase (AGP) catalyzes the substrate to produce an intermediate product, which produce chromogenic substances after enzyme catalysis. The substance has a maximum absorption peak at around 450 nm, and the AGP activity can be calculated by the OD value at 450 nm
Triglycerides were converted by enzyme to produce hydrolytic products, which were catalyzed by the enzyme to produce fluorescent substances. The triglyceride accumulation can be calculated by meas-uring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
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