Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the fluuorescence intensity of the light signal is proportional to the ATP content.
Malondialdehyde (MDA) in the degradation products of peroxidized lipids can react with thiobarbituric acid (TBA) under high temperature and acidic conditions to produce the red-brown product 3,5,5 '-trimethyloxazole-2, 4-dione (trimethine), and this substance has the maximum absorption peak at 532 nm.
Mitochondrial oxidative phosphorylation consumes oxygen to produce ATP, which provides energy for cell growth. Therefore, detection of cellular oxygen consumption is a key indicator of mitochondrial function. The kit provides a fluorescent probe, which is sensitive to oxygen. The fluorescence of the probe increases with the decrease of oxygen in a closed environment, and the oxygen consumption rate of cells is judged by detecting the change of the fluorescence value.
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