Alanine (Ala) is a non-essential amino acid, which is one of the 20 basic amino acids that make up proteins. Ala has a high content in human blood and plays an important role in human metabolic activities such as glucose-alanine cycle. It can also help the nervous system conduction and plays an important role in maintaining human health. The detection principle of this kit is as follows: alanine is decomposed by enzymes to generate NADH, which is catalyzed by electron carrier to form a chromogenic substance with WST-8. The chromogenic substance has a maximum absorption at the wavelength of 450 nm, and the Ala content in the sample is calculated by measuring the OD value at 450 nm and the standard curve.
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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