The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, which under the action of electron coupling agent, transfer electrons to WST-8 to produce the yellow product. The activity of ADH can be calculated by measuring the change of absorbance value at 450 nm.
The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD+ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Aldo-keto reductase (AKRS) is distributed in prokaryotes and eukaryotes, and there are a variety of isoforms. AKRS can catalyze the NADPH-dependent carbonyl reduction reaction to generate the corresponding alcohols. Studies have shown that AKRS are associated with a variety of cancers. The principle of this kit is that the substrate is catalyzed by AKRS, and the chromogenic substance produced by the reaction of the product and the chromogenic agent has a characteristic absorption peak at 450 nm. By detecting the amount of chromogenic substance produced per unit time, the activity of AKRS enzyme in the sample can be calculated.
Alkaline phosphatase decompose benzene disodium phosphate to produce free phenol and phosphoric acid. Phenol react with 4-aminopyrline in alkaline solution and oxidizes with potassium ferricyanide to form red quinone derivative. The enzyme activity can be calculated indirectly by measuring the OD value.
Alkaline phosphatase (ALP) is a group of cytomembrane-related enzymes with hydrolysis and transfer activity, acting on a variety of phosphate substrates. ALP is a homologous dimerase and each catalytic site contains three metal ions. There are four isozymes in humans: tissue nonspecific ALP, intestinal ALP, placental ALP and genital cell ALP.
α-Amylase in human body can not only act on the end of starch, but also act on the α-1,4 glucoside bond inside starch molecules. The degradation products are glucose, maltose and dextrin containing α-1,6 glucoside bond branch chain, which plays an important role in the digestion of polysaccharide compounds in food. Inhibition of α-amylase activity can not only reduce the digestion and absorption of starch, but also allow undigested and absorbed starch to enter the large intestine, promote gastrointestinal motility, and further reduce the occurrence of type 2 diabetes, which is an important drug target for the treatment of diabetes. The detection principle of this kit: α-Amylase catalyzes the substrate reaction to generate colored products, whose absorbance increases at 405 nm. The addition of an inhibitor suppresses the activity of α-Amylase, resulting in a reduced rate of absorbance increase. The inhibition rate can be calculated based on the absorbance difference.
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