This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse MMP-3 are added to the micro ELISA plate wells. Mouse MMP-3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse MMP-3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MMP-3. The concentration of Mouse MMP-3 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse Pg are added to the micro ELISA plate wells. Mouse Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SELP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse SELP are added to the micro ELISA plate wells. Mouse SELP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Mouse SELP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SELP. The concentration of Mouse SELP in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse T are added to the micro ELISA plate wells. Mouse T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse T in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine Cortisol are added to the micro ELISA plate wells. Porcine Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Porcine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
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