This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine T are added to the micro ELISA plate wells. Porcine T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Porcine T in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Cortisol are added to the micro ELISA plate wells. Rabbit Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rabbit Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit E2 are added to the micro ELISA plate wells. Rabbit E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rabbit E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Pg are added to the micro ELISA plate wells. Rabbit Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rabbit Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ACE2 are added to the micro ELISA plate wells. Rat ACE2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ACE2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE2. The concentration of Rat ACE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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