This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene.
Asparaginase (ASNase) is a proteolytic enzyme that was first discovered in guinea pigs and is not naturally produced in humans. Asparaginase has been widely used in the food industry, inhibiting the production of acrylamide carcinogens by hydrolyzing asparagine. Asparaginase is also used to treat various diseases due to its unique anti-tumor properties, such as acute lymphocytic leukemia, non-Hodgkin's lymphoma, leukemia meningitis, lung cancer and ovarian cancer and other tumor diseases. The detection principle of this kit is as follows: asparaginase catalyzes the substrate to generate aspartic acid, and aspartic acid is hydrolyzed under the catalytic action of the enzyme. The amount of NADH consumed by the reaction is directly proportional to the activity of asparaginase. NADH is absorbed at a wavelength of 340 nm, and the activity of asparaginase can be calculated based on the change in absorbance value per unit time.
A synthesized peptide derived from human Asparagine synthetase, corresponding to a region within N-terminal amino acids.
AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
AST enables alpha-ketoglutaric acid and aspartic acid to displace amino to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
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