The Rabbit TNF alpha yeast-derived recombinant protein is not tagged and is naturally endotoxin-free. The purity is greater than 98% as visualized by SDS-PAGE analysis. Rabbit TNF alpha applications are for cell culture, ELISA standard, and Western Blot Control. The Rabbit TNF alpha yeast-derived recombinant protein can be purchased in multiple sizes. Rabbit TNF alpha Specifications: (Molecular Weight: 17.0 kDa) (Amino Acid Sequence: SASRALSDKP LAHVVANPQV EGQLQWLSQR ANALLANGMK LTDNQLVVPA DGLYLIYSQV LFSGQGCRSY VLLTHTVSRF AVSYPNKVNL LSAIKSPCHR ETPEEAEPMA WYEPIYLGGV FQLEKGDRLS TEVNQPEYLD LAESGQVYFG IIAL (154)) (Gene ID: 100009088). For research use only. Made in the USA
The Rabbit TNFSF15 yeast-derived recombinant protein is not tagged and is naturally endotoxin-free. The purity is greater than 98% as visualized by SDS-PAGE analysis. Rabbit TNFSF15 applications are for cell culture, ELISA standard, and Western Blot Control. Rabbit TNFSF15 Specifications: (Molecular Weight: 20.4 kDa) (Amino Acid Sequence: LKGREFGPSQ QRAYMPLRAD GNKPRAHLTA VKQTPTQPLR NHFPALHWEH ELGLAFTKNR MNYTNKFLVI PESGDYFVYS QVTFRGTTSE CGVINQRRRQ TKPDSIVVVI TKVTDNYPEP AQLLTGTKSV CEMGNWFQPI YLGAMFSLEE GDKLMVNVSD VSLVDYTKED KTFFGAFLL). For research use only. Made in the USA
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TNF-α. You can calculate the concentration of Rabbit TNF-α in the samples by comparing the OD of the samples to the standard curve.
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