This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LH. You can calculate the concentration of Rat LH in the samples by comparing the OD of the samples to the standard curve.
The Rat Leukemia Inhibitory Factor (LIF) ELISA contains capture antibody, standard, and detection antibody for development of a Rat Leukemia Inhibitory Factor ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. This allows for complete flexibility to provide optimal dynamic range and sensitivity based on your sample type. Alternate names include LIF, CDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, interleukin 6 family cytokine, LIF interleukin 6 family cytokine. The quantities of components provided are not matched. Components may also be purchased separately. For research use only. Made in the USA
The Rat LIF yeast-derived recombinant protein is not tagged and is naturally endotoxin-free. The purity is greater than 98% as visualized by SDS-PAGE analysis. Rat LIF applications are for cell culture, ELISA standard, and Western Blot Control. Rat LIF yeast-derived recombinant protein can be purchased in multiple sizes. Rat LIF Specifications: (Molecular Weight: 19.8 kDa) (Amino Acid Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVAYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSNVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISAVVQAF (180)) (Gene ID: 60584). For research use only. Made in the USA
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