Glycolate oxidase catalyzes sodium glycolate substrate to form glyoxylic acid, which reacts with phenylhydrazine hydrochloride to form phenylhydrazone glyoxalate. The substance has an absorption peak at 324 nm, and its OD value is proportional to the concentration of phenylhydrazone glyoxalate in a certain range, and the amount of phenylhydrazone generated reflects the activity of glycolate oxidase.
