3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is the rate-limiting enzyme of mevalonate pathway, which is the metabolic pathway of acetyl-CoA to produce cholesterol.This detection kit can screen inhibitors of HMGR, which is an important tool for basic research of cholesterol and other related metabolic pathways. The detection principle of this kit: HMGR catalyzed substrate reaction consumes NADPH, and the absorbance decreases at 340 nm. The addition of inhibitors will inhibit HMGR enzyme activity, resulting in a decrease in the rate of absorbance decline. The inhibition rate can be calculated by measuring the change value of absorbance.
3-Hydroxy-3-methyl glutaric acyl coenzyme A reductase (hydroxy methyl glutaric acyl coenzyme A reductase, HMGR) is a hydroxy valeric acid way to control of the rate of enzyme, A hydroxy valeric acid way is acetyl CoA - produces cholesterol metabolic pathways, HMGR detection kit is cholesterol and other related metabolic pathways important means of basic research. The detection principle of this kit is as follows: HMGR catalyzes the substrate reaction to consume NADPH, causing a decrease in absorbance at 340 nm, and the activity of HMGR can be calculated by measuring its decline rate.
5'-Nucleotide enzyme (5'-NT, EC 3.1.3.5), full name is 5'-ribonucleotide phosphohydrolase, is a special phosphohydrolase that specifically hydrolyzes 5'-phosphoric acid attached to pentose in 5'-nucleotide. This enzyme is widely distributed in the cell membrane of various tissues of human and animal. Only the 5'-NT released by the tissue cells of the hepatobiliary system may enter the blood. Therefore, the source of serum 5'-NT has certain specificity, and the determination of serum 5'-NT has important value for the diagnosis of hepatobiliary diseases.
6-phosphogluconate dehydrogenase (6-PGDH) is the rate-limiting enzyme of the pentose phosphate pathway, which is responsible for the production of NADPH, energy balance, growth rate, and cell viability. 6-PGDH is also associated with a variety of tumor signaling pathways, including epidermal growth factor signaling pathway and nuclear factor E2 related factor signaling pathway. The principle of this kit is that the product generated by the 6-PGDH catalytic substrate reacts with the chromogenic agent to generate the chromogenic substance, which has the maximum absorption at 450 nm, and is calculated by measuring the OD value at 450 nm 6-PGDH enzyme activity.
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