This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Pg are added to the micro ELISA plate wells. Canine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Canine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken Cort. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken Cort are added to the micro ELISA plate wells. Chicken Cort in samples (or standards) competes with a fixed amount of Cort on the solid phase supporter for sites on the HRP linked detection antibody specific to Cort. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Chicken Cort in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken E2 are added to the micro ELISA plate wells. Chicken E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Chicken E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken E3 are added to the micro ELISA plate wells. Chicken E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Chicken E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
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