This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ApoA1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ApoA1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ApoA1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 22 nm. The OD value is proportional to the concentration of ApoA1 . You can calculate the concentration of ApoA1 in the samples by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human C5a are added to the micro ELISA plate wells. Human C5a in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human C5a.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C5a. The concentration of Human C5a in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CA19-9 are added to the micro ELISA plate wells. Human CA19-9 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CA19-9.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA19-9. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CASP3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CASP3 are added to the micro ELISA plate wells. Human CASP3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CASP3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CASP3. The concentration of Human CASP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD147. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CD147 are added to the micro ELISA plate wells. Human CD147 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CD147.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD147. The concentration of Human CD147 in the samples is then determined by comparing the OD of the samples to the standard curve.
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