This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CXCL1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CXCL1 are added to the micro ELISA plate wells. Human CXCL1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CXCL1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CXCL1. The concentration of Human CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys-C.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Cys-C. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human D2D are added to the micro ELISA plate wells. Human D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human D2D.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human D2D. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human DKK1 are added to the micro ELISA plate wells. Human DKK1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human DKK1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DKK1. The concentration of Human DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E2 are added to the micro ELISA plate wells. Human E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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