This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PDGF-BB . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PDGF-BB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PDGF-BB , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 20 nm. The OD value is proportional to the concentration of PDGF-BB . You can calculate the concentration of PDGF-BB in the samples by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PⅢNP are added to the micro ELISA plate wells. Human PⅢNP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PⅢNP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. The concentration of Human PⅢNP in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLAP/ALPP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PLAP/ALPP are added to the micro ELISA plate wells. Human PLAP/ALPP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PLAP/ALPP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLAP/ALPP. The concentration of Human PLAP/ALPP in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human POSTN/OSF-2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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