This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SELP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human SELP are added to the micro ELISA plate wells. Human SELP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human SELP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SELP. The concentration of Human SELP in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SEMA4D . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SEMA4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SEMA4D , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 14 nm. The OD value is proportional to the concentration of SEMA4D . You can calculate the concentration of SEMA4D in the samples by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sST2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human sST2 are added to the micro ELISA plate wells. Human sST2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human sST2.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sST2. The concentration of Human sST2 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFF3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TFF3 are added to the micro ELISA plate wells. Human TFF3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human TFF3.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFF3. The concentration of Human TFF3 in the samples is then determined by comparing the OD of the samples to the standard curve.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFPI. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human TFPI are added to the micro ELISA plate wells. Human TFPI in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human TFPI.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFPI. The concentration of Human TFPI in the samples is then determined by comparing the OD of the samples to the standard curve.
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