Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range.
Luciferase combines with luciferin and undergoes an oxidation reaction in the presence of ATP and oxygen, releasing fluorescence. When both luciferase and luciferin are in excess, within a certain range, the intensity of the light signal is proportional to the ATP content.
A synthesized peptide derived from human ATP citrate lyase, corresponding to a region within the internal amino acids.
Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.
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