Creatine kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate. The content of phosphocreatine was determined by colorimetric method to reflect the content of ATP.
Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry.
Adenosine Triphosphate (ATP) is a high-energy phosphate compound, which is the most direct energy source in the body, and its content is directly related to the state of energy metabolism in the body. Hexokinase (HK) catalyzes the generation of glucose and ATP into glucose-6-phosphate, and glucose-6-phosphate dehydrogenase (G6PD) further catalyzes the dehydrogenation of glucose-6-phosphate into NADPH. NADPH can react with the color developer, and the product has a characteristic absorption peak at 460 nm to determine the ATP content.
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