Malate and NAD+ form oxoacetate and NADH under the action of malate dehydrogenase, then acetyl-coa and oxoacetate form coenzyme A and citrate under the action of citrate synthetase. The content of acetyl-coA can be calculated by measuring the change of absorbance value at 340 nm
Acetyl-CoA is an essential cofactor and acyl carrier in the enzymatic acetyl transfer reaction. It can be formed by oxidative decarboxylation of pyruvate in mitochondria, oxidation of long-chain fatty acids, or oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound of the citric acid cycle. The detection principle of this kit is that Acetyl-CoA is converted into other substances under the condition of enzyme catalysis, and the resulting fluorescence is detected by fluorescence microplate reader at the maximum excitation wavelength of 535 nm and the maximum emission wavelength of 587 nm.
Acetyl-FOXO1A (Lys294) Antibody detects endogenous levels of Acetyl-FOXO1A only when acetylated at Lys294.
Acetyl-FOXO3A (Lys271) Antibody detects endogenous levels of FOXO3A only when acetylated at Lys271.
Acetyl-FOXO3A (Lys290) Antibody detects endogenous levels of FOXO3A only when acetylated at Lys290.
Acetyl-H2A.Z (Lys4/7/11/13) Antibody detects endogenous levels of Acetyl-H2A.Z only when acetylated at Lys4/7/11/13.
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